Session B27: Experimental Techniques in Biophysics
11:15 AM–2:03 PM, Monday, March 15, 2010
Room: D137
Sponsoring Unit:
DBP
Chair: Mark Reeves, George Washington University
Abstract ID: BAPS.2010.MAR.B27.4
Abstract: B27.00004 : Simultaneous multi-point laser ablation using a spatial light modulator
11:51 AM–12:03 PM
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Abstract 
Authors:
Aroshan Jayasinghe
(Dept. of Physics \& Astronomy, Vanderbilt University, Nashville, TN)
Shane Hutson
(Dept. of Physics \& Astronomy, Vanderbilt University, Nashville, TN)
Laser-microsurgery has emerged as a powerful technique for evaluating in vivo tissue mechanics; however, for incisions involving multiple pulses, only the very first pulse ablates tissue with unaltered mechanical stress; subsequent pulses ablate tissue that is recoiling from earlier ablations. To avoid this, we have developed a system for simultaneously ablating tissue at several points by using a single laser pulse shaped by a phase-only spatial light modulator (SLM). The ablating laser system is connected to a confocal microscope with a high-speed camera attachment. Using the high-speed camera and pulsed illumination, we have imaged the dynamics of multi-point ablation -- including the formation and interaction of multiple simultaneous plasmas and cavitation bubbles. We report preliminary results from simultaneous ablation of multiple spots and/or extended lines in aqueous solution, gels and fruit fly embryos.
To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2010.MAR.B27.4