Bulletin of the American Physical Society
2009 APS March Meeting
Volume 54, Number 1
Monday–Friday, March 16–20, 2009; Pittsburgh, Pennsylvania
Session Y2: Advances in Optical Microscopy |
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Sponsoring Units: DCMP Chair: David Weitz, Harvard University Room: Spirit of Pittsburgh Ballroom BC |
Friday, March 20, 2009 8:00AM - 8:36AM |
Y2.00001: Structured illumination microscopy (SIM) Invited Speaker: |
Friday, March 20, 2009 8:36AM - 9:12AM |
Y2.00002: Differential Dynamic Microscopy: a simple means to measure dynamics with a microscope Invited Speaker: Optical microscopy is an excellent tool to investigate the structure and dynamics of soft and biological materials. In this contribution we present a novel scheme to measure the dynamics of a system using an ordinary microscope [1]. This scheme is based on the spatial Fourier analysis of a time series of microscopy images, which enables us to study the relaxation of the intensity Fourier components at different spatial frequencies. This quantifies the dynamic activity of the system at different wave-vectors, giving access to information similar to the one obtained in dynamic light scattering experiments. Our technique termed Differential Dynamic Microscopy (DDM) is capable of monitoring the dynamics of both objects that are larger and smaller than the wavelength of light. The remarkable simplicity of DDM makes it suitable for the use in any laboratory that is equipped with an ordinary microscope.\\[4pt] [1] Roberto Cerbino and Veronique Trappe, ``Differential Dynamic Microscopy: Probing Wave Vector Dependent Dynamics with a Microscope,'' Phys. Rev. Lett. 100, 188102 (2008) [Preview Abstract] |
Friday, March 20, 2009 9:12AM - 9:48AM |
Y2.00003: Holographic video microscopy Invited Speaker: |
Friday, March 20, 2009 9:48AM - 10:24AM |
Y2.00004: New techniques for fluorescence background rejection in microscopy and endoscopy Invited Speaker: Confocal microscopy is a popular technique in the bioimaging community, mainly because it provides optical sectioning. However, its standard implementation requires 3-dimensional scanning of focused illumination throughout the sample. Efficient non-scanning alternatives have been implemented, among which the simple and well-established incoherent structured illumination microscopy (SIM) [1]. We recently proposed a similar technique, called Dynamic Speckle Illumination (DSI) microscopy, wherein the incoherent grid illumination pattern is replaced with a coherent speckle illumination pattern from a laser, taking advantage of the fact that speckle contrast is highly maintained in a scattering media, making the technique well adapted to tissue imaging [2]. DSI microscopy relies on the illumination of a sample with a sequence of dynamic speckle patterns and an image processing algorithm based only on an a priori knowledge of speckle statistics. The choice of this post-processing algorithm is crucial to obtain a good sectioning strength: in particular, we developed a novel post-processing algorithm based one wavelet pre-filtering of the raw images and obtained near-confocal fluorescence sectioning in a mouse brain labeled with GFP, with a good image quality maintained throughout a depth of $\sim $100 $\mu $m [3]. In the purpose of imaging fluorescent tissue at higher depth, we recently applied structured illumination to endoscopy. We used a similar set-up wherein the illumination pattern (a one-dimensional grid) is transported to the sample with an imaging fiber bundle with miniaturized objective and the fluorescence image is collected through the same bundle. Using a post-processing algorithm similar to the one previously described [3], we obtained high-quality images of a fluorescein-labeled rat colonic mucosa [4], establishing the potential of our endomicroscope for bioimaging applications. \\[4pt] \underline {Ref:} \\[0pt] [1] M. A. A. Neil \textit{et al}, Opt. Lett. 22, 1905 (1997) \\[0pt] [2] C. Ventalon \textit{et al}, Opt. Lett. \textbf{30}, 3350 (2005) \\[0pt] [3] C. Ventalon \textit{et al}, Opt. Lett. \textbf{32,} 1417 (2007) \\[0pt] [4] N. Bozinovic \textit{et al}, Opt. Express \textbf{16}, 8016 (2008) [Preview Abstract] |
Friday, March 20, 2009 10:24AM - 11:00AM |
Y2.00005: Photoactivated localization microscopy combined with single particle tracking Invited Speaker: |
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