Session H34: DNA and Protein Analysis with Nanofluidics

8:00 AM–11:00 AM, Tuesday, March 6, 2007
Colorado Convention Center Room: 404

Sponsoring Unit: DBP
Chair: Bob Austin, Princeton University

Abstract ID: BAPS.2007.MAR.H34.3

Abstract: H34.00003 : A Single--Step Photolithographic Interface for Cell-Free Gene Circuits and Active Biochips

8:48 AM–9:00 AM

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  Amnon Buxboim
    (Weizmann Institute of Science)

  Maya Bar-Dagan
    (Weizmann Institute of Science)

  Veronica Frydman
    (Weizmann Institute of Science)

  David Zbaida
    (Weizmann Institute of Science)

  Margherita Morpurgo
    (Weizmann Institute of Science)

  Roy Bar-Ziv
    (Weizmann Institute of Science)

We developed a biochip platform technology suitable for controlled cell-free gene expression at the micron scale. A new hybrid molecule, ``daisy,'' was designed and synthesized to form in a single step a bio-compatible lithographic interface on silicon dioxide. A protocol was formulated for immobilization of linear DNA molecules thousands of base pairs long on daisy-coated surfaces to submicron spatial resolution and up to high densities. On-chip protein synthesis can be obtained with dynamic range of up to four orders of magnitude and minimal nonspecific activity. En route to on-chip artificial gene circuits, a simple two-stage gene cascade was built in which the protein synthesized at the first location diffuses to regulate the synthesis of another protein at a second location. The current approach opens possibilities for laboratories not proficient in surface chemistry to design active biochips based on cell-free gene expression with applications in artificial systems and synthetic biology.

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