Session C1: Poster Session I

2:00 PM–2:00 PM, Monday, March 13, 2006
Baltimore Convention Center Room: Exhibit Hall 2:00pm - 5:00pm (DPOLY session: 11:15am - 2:15pm)

Abstract ID: BAPS.2006.MAR.C1.116

Abstract: C1.00116 : Cubic Phase Formation in Peptide/Lipid Systems

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  Brandon Scuffins
  Beth Cunningham
    (Bucknell University)

  David Wolfe
    (Lycoming College)

Previous studies have shown that the phenomena of spontaneous membrane self-assembly can be used to incorporate membrane peptides into lipid bilayers. Once a peptide is incorporated in these peptide/lipid systems they may then be crystallized through the process of \textit{in meso} crystallization. In this study, we used x-ray diffraction and $^{31}$P NMR to show that a system of dioloeoylphosphatidylethanolamine (DOPE), monoolein (MO), and DOPE with polyethylene glycol covalently attached to the headgroup (PEG-lipid) can create a system with a higher concentration of peptide incorporated into the cubic phase then previously reported. We have observed that DOPE:MO:PEG-lipid at a molar ratio of 97.5:100:2.5 naturally forms the Im3m cubic phase at room temperature. Furthermore, we found that the DOPE:MO:PEG-lipid system can incorporate a concentration of up to 25 mole {\%} peptide at room temperature. Preliminary results indicate that the lipid/peptide system requires a stable cubic phase for peptide crystallization to occur.

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