2:00 PM–2:00 PM, Monday, March 13, 2006
Baltimore Convention Center - Exhibit Hall 2:00pm - 5:00pm (DPOLY session: 11:15am - 2:15pm)
Abstract ID: BAPS.2006.MAR.C1.116
Brandon Scuffins
Beth Cunningham
(Bucknell University)
David Wolfe
(Lycoming College)
Previous studies have shown that the phenomena of spontaneous membrane self-assembly can be used to incorporate membrane peptides into lipid bilayers. Once a peptide is incorporated in these peptide/lipid systems they may then be crystallized through the process of \textit{in meso} crystallization. In this study, we used x-ray diffraction and $^{31}$P NMR to show that a system of dioloeoylphosphatidylethanolamine (DOPE), monoolein (MO), and DOPE with polyethylene glycol covalently attached to the headgroup (PEG-lipid) can create a system with a higher concentration of peptide incorporated into the cubic phase then previously reported. We have observed that DOPE:MO:PEG-lipid at a molar ratio of 97.5:100:2.5 naturally forms the Im3m cubic phase at room temperature. Furthermore, we found that the DOPE:MO:PEG-lipid system can incorporate a concentration of up to 25 mole {\%} peptide at room temperature. Preliminary results indicate that the lipid/peptide system requires a stable cubic phase for peptide crystallization to occur.
To cite this abstract, use the following reference: http://meetings.aps.org/link/BAPS.2006.MAR.C1.116