Bulletin of the American Physical Society
69th Annual Gaseous Electronics Conference
Volume 61, Number 9
Monday–Friday, October 10–14, 2016; Bochum, Germany
Session VF3: Biological Applications of Plasma II |
Hide Abstracts |
Chair: Stephan Reuter, INP Greifswald Room: 2b |
Friday, October 14, 2016 11:00AM - 11:15AM |
VF3.00001: Generation of reactive oxygen and nitrogen species and its effects on DNA damage in lung cancer cells exposed to atmospheric pressure helium/oxygen plasma jets Tae Hun Chung, Hea Min Joh, Sun Ja Kim, Ji Ye Choi, Tae-Hong Kang We investigated the effects of the operating parameters on the generation of reactive oxygen and nitrogen species (RONS) in the gas and liquid phases exposed to atmospheric pressure a pulsed-dc helium plasma jets. The densities of reactive species including OH radicals were obtained at the plasma-liquid surface and inside the plasma-treated liquids using ultraviolet absorption spectroscopy and chemical probe method. And the nitrite concentration was detected by Griess assay. The data are very suggestive that there is a strong correlation among the production of RONS in the plasmas and liquids. Exposure of plasma to cancer cells increases the cellular levels of RONS, which has been linked to apoptosis and the damage of cellular proteins, and may also indirectly cause structural damage to DNA. To identify the correlation between the production of RONS in cells and plasmas, various assay analyses were performed on plasma treated human lung cancer cells (A549) cells. In addition, the effect of additive oxygen gas on the plasma-induced oxidative stress in cancer cells was investigated. It was observed that DNA damage was significantly increased with helium/oxygen plasma compared to with pure helium plasma.. [Preview Abstract] |
Friday, October 14, 2016 11:15AM - 11:30AM |
VF3.00002: Biological decontamination of surfaces using guided ionization waves. Julien Jarrige, Clement Zaepffel Atmospheric pressure plasma jets have received an increasing attention these last ten years in various domains, including biomedical applications and decontamination. Among these technologies, guided ionization waves (also called ``plasma bullets'') are very promising because of their ability to produce a highly non-equilibrium plasma. Reactive species can be generated in the open air over a long distance during the propagation of the wave (typically: several cm), while the background gas remains at ambient temperature. A non-thermal plasma system has been developed and tested for the biological decontamination of surfaces. It consists of a dielectric barrier discharge in a helium flow driven by high voltage pulses. The propagation of the ionization wave and the spatial distribution of the species have been characterized by high speed imaging and optical emission spectroscopy. The influence of the discharge parameters on the plasma properties is investigated. Results of decontamination on several bacteria are shown, and the decontamination efficiency is compared with the plasma properties. [Preview Abstract] |
Friday, October 14, 2016 11:30AM - 11:45AM |
VF3.00003: Bactericidal active ingredient in cryopreserved plasma-treated water with the reduced-pH method for plasma disinfection Katsuhisa Kitano, Satoshi Ikawa, Yoichi Nakashima, Atsushi Tani, Takashi Yokoyama, Tomoko Ohshima For the plasma disinfection of human body, plasma sterilization in liquid is crucial. We found that the plasma-treated water (PTW) has strong bactericidal activity under low pH condition. Physicochemical properties of PTW is discussed based on chemical kinetics. Lower temperature brings longer half-life and the bactericidal activity of PTW can be kept by cryopreservation. High performance PTW, corresponding to the disinfection power of 22 log reduction (\textit{B. subtilis} spore), can be obtained by special plasma system equipped with cooling device. This is equivalent to 65{\%} H$_{\mathrm{2}}$O$_{\mathrm{2}}$, 14 {\%} sodium hypochlorite and 0.33 {\%} peracetic acid, which are deadly poison for human. But, it is deactivated soon at higher temperature (4 sec. at body temperature), and toxicity to human body seems low. For dental application, PTW was effective on infected models of human extracted tooth. Although PTW has many chemical components, respective chemical components in PTW were isolated by ion chromatography. In addition to peaks of H$_{\mathrm{2}}$O$_{\mathrm{2}}$, NO$_{\mathrm{2}}^{\mathrm{-}}$ and NO$_{\mathrm{3}}^{\mathrm{-}}$, a specific peak was detected. and only this fraction had bactericidal activity. Purified active ingredient of PTW is the precursor of HOO\textbullet , and further details will be discussed in the presentation. [Preview Abstract] |
Friday, October 14, 2016 11:45AM - 12:00PM |
VF3.00004: Mechanisms of selective antitumor action of cold atmospheric plasma David Graves, Georg Bauer Transformed (precancerous) cells are known to be subject to elimination through intercellular RONS-dependent apoptosis-inducing signaling. It is a remarkable fact that the chemical species utilized by apoptosis induction in transformed cells are essentially identical to chemical species created by cold atmospheric plasma (CAP) in aqueous solutions. The association between CAP-induced biochemistry and natural cell anti-tumor mechanisms offers the opportunity to establish a rationale for the observed successes of CAP in selectively eliminating tumor cells in vitro and in vivo. In particular, 1O2 appears to act to selectively induce apoptosis in tumor cells, and can also result in self-perpetuating, cell-to-cell apoptotic signaling. Various CAP-generated liquid phase species can react to form 1O2, thus providing a hypothetical mechanism to explain how CAP can trigger therapeutic apoptosis in tumors. The analysis of model experiments performed with defined RONS in vitro implies that CAP-derived 1O2 induces the mechanism through which CAP acts selectively against cancer cells in vitro and tumors in vivo. This hypothesis needs to be tested experimentally in order to establish its validity. [Preview Abstract] |
Friday, October 14, 2016 12:00PM - 12:15PM |
VF3.00005: Membrane Deformation and Permeabilization Caused by Microplasma Irradiation Hideki Motomura, Hidenori Nagaiwa, Kenta Yamamoto, Yugo Kido, Yoshihisa Ikeda, Susumu Satoh, Masafumi Jinno The microplasma irradiation achieves high gene taransfection efficiency and high cell survivability simultaneously. For this purpose, we have developed a special plasma source using a microcapillary electrode. However, it is not clear how the stimuli of effective factors generated by plasma, such as current, charge, field, chemical species, cause transfection. In this study, we used artificial cell which is a spherical vesicle consisting of a lipid bilayer to visualize membrane dynamics and permeabilization caused by microplasma irradiation. Dioleoyl phosphatidylcholine (DOPC) was used as phospholipid molecules forming the lipid bilayer. The artificial cells were prepared by natural swelling method. Fluorescent labeled polyethylene glycol (PEG) polymers (Nanocs, MPEG Fluorescein, MW = 1000) were encapsulated in the artificial cells. The artificial cells were exposed to the microplasma for 5~ms and 10--20\% of decrease of the dye fluorescence in the artificial cells was observed. This result suggests the outflow of the MPEG polymers through temporary poration or deformation of the lipid bilayer. The membrane deformation dynamics was directly observed with a microscope and the relationship to the polymer outflow will be shown at the conference. [Preview Abstract] |
Friday, October 14, 2016 12:15PM - 12:30PM |
VF3.00006: Investigating the cell death mechanisms in primary prostate cancer cells using low-temperature plasma treatment Deborah O'Connell, A M Hirst, J R Packer, M S Simms, V M Mann, F M Frame, N J Maitland Atmospheric pressure plasmas have shown considerable promise as a potential cancer therapy. An atmospheric pressure plasma driven with kHz kV excitation, operated with helium and oxygen admixtures is used to investigate the interaction with prostate cancer cells. The cytopathic effect was verified first in two commonly used prostate cancer cell lines (BPH-1 and PC-3 cells) and further extended to examine the effects in paired normal and tumour prostate epithelial cells cultured directly from patient tissues. Through the formation of reactive species in cell culture media, and potentially other plasma components, we observed high levels of DNA damage, together with reduced cell viability and colony-forming ability. We observed differences in response between the prostate cell lines and primary cells, particularly in terms of the mechanism of cell death. The primary cells ultimately undergo necrotic cell death in both the normal and tumour samples, in the complete absence of apoptosis. In addition, we provide the first evidence of an autophagic response in primary cells. This work highlights the importance of studying primary cultures in order to gain a more realistic insight into patient efficacy. [Preview Abstract] |
Follow Us |
Engage
Become an APS Member |
My APS
Renew Membership |
Information for |
About APSThe American Physical Society (APS) is a non-profit membership organization working to advance the knowledge of physics. |
© 2024 American Physical Society
| All rights reserved | Terms of Use
| Contact Us
Headquarters
1 Physics Ellipse, College Park, MD 20740-3844
(301) 209-3200
Editorial Office
100 Motor Pkwy, Suite 110, Hauppauge, NY 11788
(631) 591-4000
Office of Public Affairs
529 14th St NW, Suite 1050, Washington, D.C. 20045-2001
(202) 662-8700