Bulletin of the American Physical Society
66th Annual Gaseous Electronics Conference
Volume 58, Number 8
Monday–Friday, September 30–October 4 2013; Princeton, New Jersey
Session LW3: Biomedical Applications I |
Hide Abstracts |
Chair: Alexander Fridman, Drexel University Room: Nassau Room |
Wednesday, October 2, 2013 3:30PM - 4:00PM |
LW3.00001: Physics and medical applications of cold atmospheric plasma Invited Speaker: Michael Keidar Recent progress in atmospheric plasmas led to the creation of cold plasmas with ion temperature close to room temperature. Varieties of novel plasma diagnostic techniques were applied in a quest to understand physics of cold plasmas. In particular it was established that the streamer head charge is about 10$^{8}$ electrons, the electrical field in the head vicinity is about 10$^{7}$ V/m, and the electron density of the streamer column is about 10$^{19}$ m$^{3}$. We have demonstrated the efficacy of cold plasma in a pre-clinical model of various cancer types (lung, bladder, breast, head, neck, brain and skin). Both \textit{in-vitro} and\textit{ in-vivo} studies revealed that cold plasmas selectively kill cancer cells. We showed that: (a) cold plasma application selectively eradicates cancer cells in vitro without damaging normal cells. (b) Significantly reduced tumor size \textit{in viv}o. Cold plasma treatment led to tumor ablation with neighbouring tumors unaffected. These experiments were performed on more than 10 mice with the same outcome. We found that tumors of about 5mm in diameter were ablated after 2 min of single time plasma treatment. The two best known cold plasma effects, plasma-induced apoptosis and the decrease of cell migration velocity can have important implications in cancer treatment by localizing the affected area of the tissue and by decreasing metastasic development. In addition, cold plasma treatment has affected the cell cycle of cancer cells. In particular, cold plasmainduces a 2-fold increase in cells at the G2/M-checkpoint in both papilloma and carcinoma cells at $\sim$24 hours after treatment, while normal epithelial cells (WTK) did not show significant differences. It was shown that reactive oxygen species metabolism and oxidative stress responsive genes are deregulated. We investigated the production of reactive oxygen species (ROS) with cold plasma treatment as a potential mechanism for the tumor ablation observed. [Preview Abstract] |
Wednesday, October 2, 2013 4:00PM - 4:15PM |
LW3.00002: Gene Transfection Method Using Atmospheric Pressure Dielectric-Barrier Discharge Plasmas Shota Sasaki, Makoto Kanzaki, Toshiro Kaneko Gene transfection which is the process of deliberately introducing nucleic acids into cells is expected to play an important role in medical treatment because the process is necessary for gene therapy and creation of induced pluripotent stem (iPS) cells. However, the conventional transfection methods have some problems, so we focus attention on promising transfection methods by atmospheric pressure dielectric-barrier discharge (AP-DBD) plasmas. AP-DBD He plasmas are irradiated to the living cell covered with genes. Preliminarily, we use fluorescent dye YOYO-1 instead of the genes and use LIVE/DEAD Stain for cell viability test, and we analyze the transfection efficiency and cell viability under the various conditions. It is clarified that the transfection efficiency is strongly dependence on the plasma irradiation time and cell viability rates is high rates (\textgreater 90 {\%}) regardless of long plasma irradiation time. These results suggest that ROS (Reactive Oxygen Species) and electric field generated by the plasma affect the gene transfection. In addition to this (the plasma irradiation time) dependency, we now investigate the effect of the plasma irradiation under the various conditions. [Preview Abstract] |
Wednesday, October 2, 2013 4:15PM - 4:30PM |
LW3.00003: Plasma-activated medium induced apoptosis on tumor cells Masaru Hori, Hiromasa Tanaka, Masaaki Mizuno, Kae Nakamura, Hiroaki Kajiyama, Keigo Takeda, Kenji Ishikawa, Hiroyuki Kano, Fumitaka Kikkawa The non-equilibrium atmospheric pressure plasma (NEAPP) has attracted attention in cancer therapy. In this study, the fresh medium was treated with our developed NEAPP, ultra-high electron density (approximately 2 $\times$ 10$^{16}$ cm$^{-3}$) [1,2]. The medium called the plasma-activated medium (PAM) killed not normal cells but tumor cells through induction of apoptosis. Cell proliferation assays showed that the tumor cells were selectively killed by the PAM. Those cells induced apoptosis using an apoptotic molecular marker, cleaved Caspase3/7. The molecular mechanisms of PAM-mediated apoptosis in the tumor cells were also found that the PAM downregulated the expression of AKT kinase, a marker molecule in a survival signal transduction pathway. These results suggest that PAM may be a promising tool for tumor therapy by downregulating the survival signals in cancers.\\[4pt] [1] M. Iwasaki et al. Appl. Phys. Lett. 92, 081503 (2008);\\[0pt] [2] H. Tanaka, et al. Plasma Medicine 1(3-4) 265-277 (2011). [Preview Abstract] |
Wednesday, October 2, 2013 4:30PM - 4:45PM |
LW3.00004: DNA damage in oral cancer cells induced by nitrogen atmospheric pressure plasma jets Xu Han, Matej Klas, Yueying Liu, M. Sharon Stack, Sylwia Ptasinska The nitrogen atmospheric pressure plasma jet (APPJ) has been shown to effectively induce DNA double strand breaks in SCC-25 oral cancer cells. The APPJ source constructed in our laboratory consists of two external electrodes wrapping around a quartz tube and nitrogen as a feed gas and operates based on dielectric barrier gas discharge. Generally, it is more challenging to ignite plasma in N2 atmosphere than in noble gases. However, this design provides additional advantages such as lower costs compared to the noble gases for future clinical operation. Different parameters of the APPJ configuration were tested in order to determine radiation dosage. To explore the effects of delayed damage and cell self-repairing, various incubation times of cells after plasma treatment were also performed. Reactive species generated in plasma jet and in liquid environment are essential to be identified and quantified, with the aim of unfolding the mystery of detailed mechanisms for plasma-induced cell apoptosis. Moreover, from the comparison of plasma treatment effect on normal oral cells OKF6T, an insight to the selectivity for cancer treatment by APPJ can be explored. All of these studies are critical to better understand the damage responses of normal and abnormal cellular systems to plasma radiation, which are useful for the development of advanced plasma therapy for cancer treatment at a later stage. [Preview Abstract] |
Wednesday, October 2, 2013 4:45PM - 5:00PM |
LW3.00005: APNTP Inactivation of MS2 Bacteriophage: Effect of operating parameters on virucidal activity Nid'a Alshraiedeh, Mahmoud Alkawareek, Sean Gorman, William Graham, Brendan Gilmore Atmospheric pressure non-thermal plasmas (APNTP) provide a promising alternative method for surface decontamination. Norovirus is globally the most common etiological agent of acute non-bacterial gastroenteritis outbreaks. APNTP have proven to be effective in inactivation of MS2 bacteriophages, widely employed as surrogate for human norovirus. Here we explore the optimization of a helium-based kHz APNTP by varying the oxygen concentration (from 0 to 0.75{\%}) in the feed gas and the operating frequency (from 10 to 40 kHz). It has been established that both these changes increase the reactive oxide concentration in the plume and we see a correlation between both increasing oxygen concentration and operating frequency and reduction in survival density of treated bacteriophages. For example increasing the O$_{2}$ concentration from 0 to 0.5 to 0.75{\%} increased the log reduction from 4.98 to 5.93 to 7.06, respectively. These results will be discussed in the context of recent studies where singlet delta oxygen was shown to cause MS2 phage inactivation.\footnote{Q T Algwari, PhD Thesis QUB (2011).}$^,$\footnote{E M Hotze et al \textit{Environ. Sci. Technol.} 43(17): 6639-6645.} [Preview Abstract] |
Wednesday, October 2, 2013 5:00PM - 5:15PM |
LW3.00006: Influence of Uniform and Non-Uniform Regimes of Nanosecond-Pulsed Dielectric Barrier Discharges on Intracellular Biochemical Processes in 10T1/2 Cells Abraham Lin, Natalie Shainsky, Daynna Park, Theresa Freeman, Danil Dobrynin, Alexander Fridman, Vandana Miller, Gregory Fridman Before investigating plasma effects on differentiation, plasma regime limiting cell death must first be determined. We report the effects of dose on cell viability and compare with dose rates. Results allow for the safe operation of plasma in a regime where nanosecond pulsed DBD on mesenchymal stem cells (MSCs) promote differentiation with limited cell death. After optimizing plasma parameters, discharge energy and uniformity effects on differentiation in MSCs can be studied. We also report the effects of direct and indirect treatment on cellular behavior. C3H-10T1/2 MSCs were treated in 12well plates and stained with two fluorescent markers: MitoSOX Red, which detects O2- and propidium iodide, which is a live/dead stain. Dose vs dose rate effects were investigated, and direct vs indirect treatments were performed with nano-pulses at varying doses while keeping power and electric field the same. We show dependence of cell death with dose and not dose rate. Additionally, we demonstrated that direct treatment elicits greater ROS generation compared to that of indirect. A correlation of cell death and ROS generation with treatment dose is required to find optimal operating parameters for nano-plasma treatment of stem cells. [Preview Abstract] |
Wednesday, October 2, 2013 5:15PM - 5:30PM |
LW3.00007: Growth control of budding yeast cells by oxygen-radical treatment Takayuki Ohta, Hiroshi Hashizume, Masafumi Ito, Keigo Takeda, Masaru Hori Microorganisms respond to stimuli or stresses such as chemicals, nutrition, pressure, heat and so on. Those stimuli lead to cell activation, inactivation or cell death, such as apoptosis and necrosis. Reactive oxygen species possibly affect cell growth as well as inactivation depending on stimuli. In this study, we investigated effects of oxygen-radical treatment on not only inactivation but also promoted/repressed cell growth of budding yeast by varying dose of atomic oxygen produced from an atmospheric-pressure oxygen radical source. Dose of atomic oxygen was estimated based on treatment time and oxygen flux. Cell growth was promoted with atomic oxygen between 0 and 2.1$\times$10$^{19}$ cm$^{-2}$ dose of atomic oxygen. The treated cells were grown about 10 percent more proliferously than the control cells. Moreover, 64 percent of the treated cells to the control ones were inactivated at more than 4.2$\times$10$^{20}$ cm$^{-2}$. These results, therefore, suggested that atomic-oxygen dose had a potential to control mitotic promotion and repression, and inactivation of yeast cell growth. [Preview Abstract] |
Follow Us |
Engage
Become an APS Member |
My APS
Renew Membership |
Information for |
About APSThe American Physical Society (APS) is a non-profit membership organization working to advance the knowledge of physics. |
© 2024 American Physical Society
| All rights reserved | Terms of Use
| Contact Us
Headquarters
1 Physics Ellipse, College Park, MD 20740-3844
(301) 209-3200
Editorial Office
100 Motor Pkwy, Suite 110, Hauppauge, NY 11788
(631) 591-4000
Office of Public Affairs
529 14th St NW, Suite 1050, Washington, D.C. 20045-2001
(202) 662-8700