Bulletin of the American Physical Society
65th Annual Gaseous Electronics Conference
Volume 57, Number 8
Monday–Friday, October 22–26, 2012; Austin, Texas
Session UF1: Biological and Biomedical Applications of Plasmas II |
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Chair: Bill Graham, Queen's University Belfast Room: Classroom 107 |
Friday, October 26, 2012 9:00AM - 9:15AM |
UF1.00001: Generation and characteristic survey of atmospheric-pressure dry, vapor, mist plasma jet using high-frequency high-voltage power supply Norimitsu Takamura, Douyan Wang, Takao Namihira, Hidenori Akiyama Atmospheric-pressure plasma jet has attracted in the various fields for example surface treatment of materials, bacterial killing and so on. The reasons why the plasma used these applications are because it is a non-thermal, high pressure, uniform glow plasma discharge that produces a high velocity effluent stream of highly reactive chemical species. While passing through the plasma, the feed gas becomes excited, dissociated or ionized by electron impact. Once the gas exits, the discharge volume, ions and electrons are rapidly lost by recombination, but the fast-flowing effluent still contains neutral metastable species and radicals. In our previous study, GFP-7R proteins were promoted delivering into the HeLa cells by dry plasma jet. In this case, we irradiated dry plasma jet only the surface of cell suspension. Therefore, it may be expected that raising the ratio of surface area/volume exposed to plasma by to mist the cell suspension causes further improvement of protein transduction efficiency by irradiating plasma. In this study, we investigated the optimal driving parameters of the plasma jets. The length of dry, vapor, and mist plasma jets and the generated chemical species of each plasma jet will be introduced at the conference. [Preview Abstract] |
Friday, October 26, 2012 9:15AM - 9:30AM |
UF1.00002: Feed gas humidity introduced into a MHz atmospheric pressure argon plasma jet affects plasma-generated species and plasma-treated human skin cells J\"orn Winter, Kristian Wende, Malte U. Hammer, Helena Tresp, Sylvain Iseni, Mario D\"unnbier, Kai Masur, Klaus-Dieter Weltmann, Stephan Reuter It is known, that gas humidity is an important parameter in plasma treatment of biological systems under ambient conditions. However, humidity in the feed gas of an atmospheric pressure plasma jet is even more crucial than ambient humidity since humid working gas is transported through the active plasma zone and the water molecules become dissociated. The so produced oxygen/hydrogen species are significant for the active plasma component composition. In this work the effect of feed gas humidity on the plasma, on plasma-treated cell growth medium (RPMI) and subsequently on human skin cells is investigated. It is shown, that already small concentrations of humidity ($<$1000 ppm) induce changes in the optical emission spectrum of the effluent, increase H$_{2}$O$_{2}$ concentration in liquid cell growth medium and inhibit human skin cell proliferation. [Preview Abstract] |
Friday, October 26, 2012 9:30AM - 9:45AM |
UF1.00003: Modeling of inactivation of surface borne microorganisms occurring on seeds by cold atmospheric plasma (CAP) Anindita Mitra, Y.-F. Li, T. Shimizu, Tobias Kl\"ampfl, J.L. Zimmermann, G.E. Morfill Cold Atmospheric Plasma (CAP) is a fast, low cost, simple, easy to handle technology for biological application. Our group has developed a number of different CAP devices using the microwave technology and the surface micro discharge (SMD) technology. In this study, FlatPlaSter2.0 at different time intervals (0.5 to 5 min) is used for microbial inactivation. There is a continuous demand for deactivation of microorganisms associated with raw foods/seeds without loosing their properties. This research focuses on the kinetics of CAP induced microbial inactivation of naturally growing surface microorganisms on seeds. The data were assessed for log- linear and non-log-linear models for survivor curves as a function of time. The Weibull model showed the best fitting performance of the data. No shoulder and tail was observed. The models are focused in terms of the number of log cycles reduction rather than on classical D-values with statistical measurements. The viability of seeds was not affected for CAP treatment times up to 3 min with our device. The optimum result was observed at 1 min with increased percentage of germination from 60.83\% to 89.16\% compared to the control. This result suggests the advantage and promising role of CAP in food industry. [Preview Abstract] |
Friday, October 26, 2012 9:45AM - 10:00AM |
UF1.00004: Influence of plasma-treatments on the structure, superstructure, and function of membrane lipids Malte U. Hammer, Enrico Forbrig, Klaus-Dieter Weltmann, Stephan Reuter Every cell, eu- or prokaryotic, has a membrane as an interface to the environment. Every substance that is applied from outside the cell has to interact with it. This includes plasma-generated reactive species in the liquid cell environment created by plasma-treatment. By the Singer and Nicolson model, proteins are embedded in a lipid bilayer. Proteins are the functional elements, lipids are the structural elements. Due to the amphiphilic nature of the lipids, they form (super-) structures in an aqueous environment. The exact superstructure is determined by a structural parameter of the lipid, its shape. Here, we show experiments on lipids by fluorophore-based liposome assays and raman spectroscopy. The results show a membrane-activity of plasma-born reactive species against lipids and lipid structures. Based on this results and literature, we propose a model for a lesion-forming mechanism in membranes of some reactive species created by plasma-treatment. It is based on a hydrophobic-hydrophilic mismatch due to lipid peroxidization induced by reactive species generated in liquids by plasma-treatment. [Preview Abstract] |
Friday, October 26, 2012 10:00AM - 10:15AM |
UF1.00005: Effect of Plasma Pretreatments on the Bio-adhesive Functionalized by Biomimetic Catechol Groups to Human Dentin Sangbae Lee, KwangMahn Kim, KyoungNam Kim Plasma pretreatments have been introduced for modifying the surface chemistry of biomaterials. In an effort to improve the strength of the human dentin/bio-adhesive joint, oxygen plasma pretreatments to the bio-adhesive were investigated. Plasma treatments were carried out using custom-built and low pressure. Dentin were treated with plasma and used to prepare lap shear tests. Bio-adhesives were prepared synthesizing dopamine methacrylamide (DMA) monomer. DMA were copolymerized with 2-methoxyethylacrylate (MEA) by free radical polymerization. Proton nuclear magnetic resonance ($^{1}$H-NMR) and Gel permeation chromatography (GPC) analysis on samples of synthesized $p$(DMA-\textit{co}-MEA) was performed to confirm that the resulting materials had the desired chemical structure. The effects of plasma pretreatments on surface chemistry were studied using Fourier transform infrared analysis (FTIR), and contact angle measurements. Oxygen plasma pretreatments enhanced adhesive strength by oxidizing of the catechol residue and creating a cross-linking as compared with control group. Furthermore plasma pretreatments lead to increase hydrophilicity of copolymers. Prospectively, the great potential of advanced technology in creation of the ``Plasma pretreatment to the DOPA adhesives'' would lead to the development of versatile method for coating to medial devices as well as dentin bonding. [Preview Abstract] |
Friday, October 26, 2012 10:15AM - 10:30AM |
UF1.00006: Treatment of oral cancer cells with nonthermal atmospheric pressure plasma jet James Yurkovich, Xu Han, Benjamin Coffey, Matej Klas, Sylwia Ptasinska Non-thermal atmospheric pressure plasmas are specialized types of plasma that are proposed as a new agent to induce death in cancer cells. The experimental phase of this study will test the application of such plasma to SCC-25 oral cancer cells to determine if it is possible to induce apoptosis or necrosis. Different sources are used on the cells to find a configuration which kills cancer cells but has no effect on normal cells. The sources have been developed based on the dielectric barrier discharge between two external electrodes surrounding a dielectric tube; such a configuration has been shown to induce breaks in DNA strands. Each configuration is characterized using an optical emission spectrophotometer and iCCD camera to determine the optimal conditions for inducing cell death. The cells are incubated after irradiation with plasma, and cell death is determined using microscopy imaging to identify antibody interaction within the cells. These studies are important for better understanding of plasma species interactions with cancer cells and mechanisms of DNA damage and at latter stage they will be useful for the development of advanced cancer therapy. [Preview Abstract] |
Friday, October 26, 2012 10:30AM - 10:45AM |
UF1.00007: The influence of the air plasma jet on early adherent events of L929 fibroblasts on cell culture polystyrene plate Jung-Hwan Lee, Jae-Sung Kwon, Ji-yeon Om, Yong-Hee Kim, Eun-Ha Choi, Kwang-Mahn Kim, Kyoung-Nam Kim Recently, atmospheric pressure plasma was applied to biological field. The aim of this study was to identify whether the air plasma jet increases fibroblast early attachment under moving motion on the cell culture polystyrene plate. Polystyrene plate was treated with plasma jet using compressed air. After 2 minutes of treatment, L929 was seeded on polystyrene plate as well as on untreated plate. Cells were allowed to attach for 4 hours under 70 RPM. FE-SEM, confocal microscopy and RT-PCR were used to evaluate characters of cells. The results suggested that plasma treatment on the polystyrene plate altered surface energy without change of roughness. In occasion of treatment plate, attached L292 were significantly found but not found on untreated surface. Also, despite the small area of treated center by the flame of the plasma jet, cells were also attached on round surface of the area covered by the flame, which suggests that the effect was not only due to the jet flame but perhaps due to the jet interacting with surrounding atmosphere. In the light of this study, the air plasma jet could be useful for early attachment of L292 on the polystyrene plate under moving motion and can be applied to biomaterials. [Preview Abstract] |
Friday, October 26, 2012 10:45AM - 11:00AM |
UF1.00008: Effect of non-thermal atmospheric pressure plasma jet on human breast cancer cells Shahriar Mirpour, Maryam Nikkhah, Somaye Pirouzmand, Hamid Reza Ghomi Nowadays, Non-thermal plasma enjoy a wide range of applications in biomedical fields such as Sterilization, Wound healing, Cancer treatment and \textit{etc}. The aim of this paper is to study the effect of non-thermal atmospheric pressure plasma jet on breast cancer (MCF-7) cells. In this regard the effect of plasma on death of the cancer cells are explored experimentally. The plasma in this discharge is created by pulsed dc high voltage power supply with repetition rate of several tens of kilohertz which led to the inductively coupled plasma. The pure helium gas were used for formation of the plasma jet. MTT assay were used for quantification of death cells. The results showed that the cells death rate increase with plasma exposure time. This study confirm that plasma jet have significant effect on treatment of human breast cancer cells. [Preview Abstract] |
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